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pGreenII 62-SK载体质粒基本信息
pGreenII 62-SK质粒图谱载体图谱和pGreenII 62-SK载体序列质粒序列多克隆位点信息
pGreenII 62-SK质粒载体简介
pGreenII 62-SK质粒序列载体序列
LOCUS pgreenII 62-SK.d 3347 bp ds-DNA circular SYN 30-JAN-2023 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3347) AUTHORS biofeng.com TITLE Direct Submission JOURNAL Exported Monday, Jan 30, 2023 from SnapGene 4.3.6 to Vector NTI(R) format https://www.snapgene.com COMMENT VNTDATE|1011744000| COMMENT VNTDBDATE|1011744000| COMMENT VNTNAME|pgreenII 62-SK.dna| COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers misc_feature 542..564 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" promoter 638..982 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 992..1099 /label=MCS /note="pBluescript multiple cloning site" primer_bind 1016..1032 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1066..1082) /label=KS primer /note="common sequencing primer, one of multiple similar variants" polyA_signal 1153..1329 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature 1339..1363 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(1454..2042) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2213..3028) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" rep_origin join(3319..3347,1..407) /label=pSa ori /note="origin of replication from bacterial plasmid pSa" ORIGIN 1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc 61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg 121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg 181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc 241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat 301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac 361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc 421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa 481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc 541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa 601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa 661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag 721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc 781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg 841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg 901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa 961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag 1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct 1081 cgaggggggg cccggtacca attcggtacg ctgaaatcac cagtctctct ctacaaatct 1141 atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag ggaaattagg 1201 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta 1261 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa 1321 atccagatcc actagccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt 1381 ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1441 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1501 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1561 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1621 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1681 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1741 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1801 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1861 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 1921 tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg 1981 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 2041 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 2101 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 2161 aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattagaaaa 2221 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt 2281 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg 2341 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt 2401 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg 2461 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac 2521 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag 2581 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc 2641 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta 2701 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag 2761 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga 2821 ccatctcatc tgtaacaaca ttggcaacgc tacctttgcc atgtttcaga aacaactctg 2881 gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc 2941 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc 3001 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag 3061 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt 3121 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca 3181 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact 3241 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg 3301 gggcgattca ggcgatcccc atccaacagc ccgccgtcga gcgggct //
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pGreenII 62-SK质粒载体应用举例
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